| Rank | Song | Count | Graph |
|---|
| 1 | Somebody to Love | 2 |  |
| 2 | (I've Had) The Time of My Life [Glee Cast Version] | 1 | |
| 3 | (You're) Having My Baby | 1 | |
| 4 | A Hard Day's Night | 1 | |
| 5 | A House Is Not a Home | 1 | |
| 6 | ABC | 1 | |
| 7 | Afternoon Delight (feat. John Stamos) | 1 | |
| 8 | All You Need Is Love | 1 | |
| 9 | Alone (feat. Kristin Chenoweth) | 1 | |
| 10 | And I Am Telling You I'm Not Going | 1 | |
| 11 | Animal | 1 | |
| 12 | Any Way You Want It / Lovin' Touchin' Squeezin' | 1 | |
| 13 | As If We Never Said Goodbye | 1 | |
| 14 | As Long As You're There | 1 | |
| 15 | Baby | 1 | |
| 16 | Bad Romance (Glee Cast Version) | 1 | |
| 17 | Beautiful | 1 | |
| 18 | Bella Notte | 1 | |
| 19 | Beth (Glee Cast Version) | 1 | |
| 20 | Billionaire | 1 | |
| 21 | Bills, Bills, Bills | 1 | |
| 22 | Blackbird | 1 | |
| 23 | Bohemian Rhapsody (feat. Jonathan Groff) | 1 | |
| 24 | Born This Way | 1 | |
| 25 | Bust a Move | 1 | |
| 26 | Bust Your Windows | 1 | |
| 27 | Can't Fight This Feeling | 1 | |
| 28 | Candles | 1 | |
| 29 | Constant Craving | 1 | |
| 30 | Control | 1 | |
| 31 | Crush | 1 | |
| 32 | Dancing Queen | 1 | |
| 33 | Dancing With Myself | 1 | |
| 34 | Defying Gravity | 1 | |
| 35 | Do Ya Think I'm Sexy? | 1 | |
| 36 | Don't Make Me Over | 1 | |
| 37 | Don't Rain On My Parade | 1 | |
| 38 | Don't Stand So Close to Me (Young Girl) | 1 | |
| 39 | Don't Stop | 1 | |
| 40 | Don't Stop Believin' | 1 | |
| 41 | Don't Stop Believin' (Regionals Version) | 1 | |
| 42 | Don't You Want Me | 1 | |
| 43 | Dream On [feat. Neil Patrick Harris] | 1 | |
| 44 | Dreams [feat. Kristin Chenoweth] | 1 | |
| 45 | Drive My Car | 1 | |
| 46 | Edge of Glory | 1 | |
| 47 | Empire State of Mind | 1 | |
| 48 | Endless Love | 1 | |
| 49 | Faithfully | 1 | |
| 50 | Fat Bottomed Girls | 1 | |
| 51 | Firework | 1 | |
| 52 | Fix You | 1 | |
| 53 | Forever Young | 1 | |
| 54 | Forget You (Glee Cast Version) [feat. Gwyneth Paltrow] | 1 | |
| 55 | Get Back | 1 | |
| 56 | Get It Right | 1 | |
| 57 | Girls Just Want to Have Fun | 1 | |
| 58 | Give Up the Funk (Glee Cast Version) | 1 | |
| 59 | Gives You Hell | 1 | |
| 60 | Glory Days | 1 | |
| 61 | Go Your Own Way | 1 | |
| 62 | Gold Digger | 1 | |
| 63 | Good Riddance (Time of Your Life) | 1 | |
| 64 | Got To Get You Into My Life (Glee Cast Version) | 1 | |
| 65 | Hate On Me | 1 | |
| 66 | Hello [feat. Jonathan Groff] | 1 | |
| 67 | Hello, Goodbye | 1 | |
| 68 | Help | 1 | |
| 69 | Here Comes the Sun (feat. Demi Lovato) | 1 | |
| 70 | Here's to Us | 1 | |
| 71 | Hey Jude | 1 | |
| 72 | Hey, Soul Sister | 1 | |
| 73 | Home [feat. Kristin Chenoweth] | 1 | |
| 74 | Hot for Teacher | 1 | |
| 75 | I Dreamed a Dream [feat. Idina Menzel] | 1 | |
| 76 | I Feel Pretty / Unpretty | 1 | |
| 77 | I Saw Her Standing There | 1 | |
| 78 | I Want to Hold Your Hand | 1 | |
| 79 | I Was Here | 1 | |
| 80 | I Won't Give Up | 1 | |
| 81 | I'll Remember | 1 | |
| 82 | I'll Stand By You | 1 | |
| 83 | I'm Not Gonna Teach Your Boyfriend How to Dance With You | 1 | |
| 84 | Imagine | 1 | |
| 85 | Isn't She Lovely | 1 | |
| 86 | It's Not Unusual | 1 | |
| 87 | Jump | 1 | |
| 88 | Just the Way You Are (Glee Cast Version) | 1 | |
| 89 | Keep Holding On | 1 | |
| 90 | Kiss (feat. Gwyneth Paltrow) | 1 | |
| 91 | Lady Is a Tramp | 1 | |
| 92 | Landslide (feat. Gwyneth Paltrow) | 1 | |
| 93 | Last Friday Night | 1 | |
| 94 | Lean On Me | 1 | |
| 95 | Let It Be | 1 | |
| 96 | Light Up the World | 1 | |
| 97 | Loser | 1 | |
| 98 | Loser Like Me | 1 | |
| 99 | Lucky (Glee Cast Version) | 1 | |
| 100 | Man In the Mirror | 1 | |